Application
RPMI 1640 Medium was developed at Roswell Park Memorial
Institute in 1966 by Moore and his co-workers. A modification of McCoy′s
5A Medium, it was formulated to support lymphoblastoid cells in
suspension culture, but it has since been shown to support a wide
variety of cells that are anchorage dependent. Originally intended to be
used with a serum supplement, RPMI 1640 has been shown to support
several cell lines in the absence of serum. It has also been widely used
in fusion protocols and in the growth of hybrid cells.
RPMI-1640 Medium is used for the following applications:
• used
in cell culture medium (i.e. Murine thymoma EL-4 cells were cultured in
RPMI 1640 medium supplemented with other components)1
• used for the mycobacterial infection of splenocytes (in antibiotic-free RPMI 1640 medium)2
• used for cell isolation and culture3,4
• used in culture medium (as one of the component) during lymphocyte separation and culture, ELISPOT Assay, Ex Vivo proliferation assay5
• used in medium for gp39 gene expression and CD40-Immunoglobuin binding assays6
• used for the preparation of antifungal agents7,8
Quantity
Formulated to contain 10.4 grams of powder per liter of medium.
Reconstitution
Supplement with 2.0 g/L sodium bicarbonate.
General description
Roswell Park Memorial Institute medium is commonly known as
RPMI. It is a form of medium used in cell culture and tissue culture.
This medium contains phosphate and is formulated for use in a 5% carbon
dioxide atmosphere. It is used for the growth of human lymphoid cells.
Protocols & Applications
ECACC Handbook-Fundamental Techniques in Cell Culture
Cryostor Cryopreservation Video Protocol
Mycoplasma PCR Detection Kit Video Protocol