Poly-L-lysine solution

mol wt 150,000-300,000, 0.01%, sterile-filtered, BioReagent, suitable for cell culture

Synonym: PDL Solution

• CAS Number 25988-63-0

•  MDL number MFCD00165424

•  eCl@ss 32160406

• Properties

  Related Categories	Amino Acids, Attachment Factors, Attachments Factors, Biochemicals and Reagents, Cell Culture, Core Bioreagents, Life Science Reagents for Cell Culture, Polyamino Acids, Polylysine and Polyornithine Attachment Factors, Reagents and Supplements, Research Essentials
  Quality Level	PREMIUM
  sterility	sterile-filtered
  product line	BioReagent
  mol wt	mol wt 150,000-300,000
  concentration	0.01%
  impurities	endotoxin, tested
  suitability	suitable for cell culture
  storage temp.	2-8°C

  Description

  Components

  Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

  Application

  Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

  Biochem/physiol Actions

  Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

  Preparation Note

  This product is supplied as a 0.01% sterile filtered solution in water, using a molecular weight of 150 - 300 kDa. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.