Frequently Asked Questions
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Analysis Note
Endotoxin and hemoglobin tested
Linkage
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Application
FBS sourced from USDA approved non-USA countries is used in a
broad range of cell culture applications. FBS provides many non-defined
growth promoting and survival enhancing factor to cells in culture.
Fetal Bovine Serum was used for the following cell cultures:
• Primary
Culture Human Macrophages (mononuclear cells were re-suspended in
Roswell Park Memorial Institute (RPMI) medium containing fetal bovine
serum (FBS) along with other components).1
• Tissue Culture (MEFs were cultured in DMEM supplemented with FBS along with components).2
• Isolation and 3D culture of primary mammary epithelial organoids3
• Cell culture and medium (HUVECs were grown in Endothelial Cell Growth Medium-2 supplemented with FBS and growth factors).4
General description
Fetal bovine serum (FBS) or fetal calf serum is the blood
fraction remaining after the natural coagulation of blood. It comes from
the blood drawn from a bovine fetus via a closed system of collection
at the slaughterhouse. It is the most widely used serum-supplement for
the in vitro cell culture of eukaryotic cells.
Suitability
USDA Approved Sourcing refers to FBS produced from blood
collected in countries outside of the United States that have been
approved by the USDA (United States Department of Agriculture) for
export of beef products into the United States. Eligible countries for
export into the U.S. include Australia, Canada, Chile, Costa Rica,
Honduras, Iceland, Japan, Mexico, New Zealand, Nicaragua, Uruguay.
Foreign serum is tested to ensure the absence of exotic viruses before
release by the USDA.
Protocols & Applications
Cryostor Cryopreservation Video Protocol
Mycoplasma PCR Detection Kit Video Protocol